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首頁 > 細(xì)胞 > 人源細(xì)胞系 > 人乳腺癌細(xì)胞(MDA-MB-468)
人乳腺癌細(xì)胞(MDA-MB-468)
￥800.00元
細(xì)胞貨號(hào)： CL0211
細(xì)胞種屬： 人源細(xì)胞系
細(xì)胞別名： MDA-MB 468; MDA-MB468; MDAMB468; MDA-468; MDA468; MB468; MD Anderson-Metastatic Breast-468
發(fā)貨規(guī)格： 1×10?
供體信息： 51歲黑人女性
腫瘤類型： 乳腺癌
組織來源： 乳腺
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背景信息
  • 種屬： 人源細(xì)胞系
  • 組織來源： 乳腺
  • 腫瘤類型： 乳腺癌
  • 供體信息： 51歲黑人女性
  • 背景描述： 成立于1977年，由一名51歲浸潤性乳腺癌女性的胸腔積液組成;文獻(xiàn)中描述細(xì)胞在密碼子273處攜帶p53點(diǎn)突變，導(dǎo)致Arg-His取代和擴(kuò)增的EGFR;三陰性
培養(yǎng)建議
  • 生長形態(tài)： 上皮細(xì)胞樣
  • 生長特性： 貼壁
  • 培養(yǎng)條件： L15+10%FBS+1%P/S+10ug/ml胰島素+15ug/ml谷胱甘肽
  • 凍存條件： 90%FBS+10%DMSO
  • 血清使用等級(jí)： 標(biāo)準(zhǔn)
  • 傳代方式： 胰酶消化
  • 倍增時(shí)間： 30~40h
  • 傳代比例： 胰酶消化
  • 換液頻率： 每周2~3次
  • 生物安全等級(jí)： 1
  • 培養(yǎng)注意事項(xiàng)：
其他信息
  • 蛋白表達(dá)： Epidermalgrowthfactor(EGF);transforminggrowthfactoralpha(TGFalpha)。蛋白質(zhì)印跡中無ER、PR、AR和HER2證實(shí)三陰性
  • 同工酶： AK-1,1；ES-D,1；G6PD,A；GLO-I,1-2；Me-2,1-2；PGM1,1；PGM3,2
  • 致瘤性： Yes, in nude mice inoculated subcutaneously with 10(7) cells.(Tumors developed within 21 days at 100% frequency (5/5).)
  • 抗原表達(dá)： Blood Type AB;HLA Aw23,Aw30,B27,Bw35,Cw2,Cw4 (patient)
  • 病毒： EBV-,HBV-,HCV-,HIV-1-,HIV-2-,HTLV-I/-II-,MLV-
  • 核型： modal number = 64; range = 60 to 67.The cell line is aneuploid human, presumably female (X, abnormal X) with most chromosome counts in the hypotriploid range.; Normal chromosomes X, N2, N3, N7, N8, N10, and N22 are clearly under-represented due to their involvement in the formation of the many marker (19) chromosomes present in this cell line.; A normal chromosome N1 (or two) is identified in each karyotype, but, in addition, regions of chromosome N1 are also present in five different marker chromosomes.; Variation is evident in the normal and marker chromosome copy number from karyotype to karyotype.
人乳腺癌細(xì)胞
Catalog No CL0211
Product information
  • 蛋白表達(dá)： Epidermalgrowthfactor(EGF);transforminggrowthfactoralpha(TGFalpha)。蛋白質(zhì)印跡中無ER、PR、AR和HER2證實(shí)三陰性
  • 同工酶： AK-1,1；ES-D,1；G6PD,A；GLO-I,1-2；Me-2,1-2；PGM1,1；PGM3,2
  • 致瘤性： Yes, in nude mice inoculated subcutaneously with 10(7) cells.(Tumors developed within 21 days at 100% frequency (5/5).)
  • 抗原表達(dá)： Blood Type AB;HLA Aw23,Aw30,B27,Bw35,Cw2,Cw4 (patient)
  • 病毒： EBV-,HBV-,HCV-,HIV-1-,HIV-2-,HTLV-I/-II-,MLV-
  • 核型： modal number = 64; range = 60 to 67.The cell line is aneuploid human, presumably female (X, abnormal X) with most chromosome counts in the hypotriploid range.; Normal chromosomes X, N2, N3, N7, N8, N10, and N22 are clearly under-represented due to their involvement in the formation of the many marker (19) chromosomes present in this cell line.; A normal chromosome N1 (or two) is identified in each karyotype, but, in addition, regions of chromosome N1 are also present in five different marker chromosomes.; Variation is evident in the normal and marker chromosome copy number from karyotype to karyotype.
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