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首頁 > 細(xì)胞 > 人源細(xì)胞系 > 人膀胱移行細(xì)胞乳頭瘤細(xì)胞(RT4)
人膀胱移行細(xì)胞乳頭瘤細(xì)胞(RT4)
￥800.00元
細(xì)胞貨號： CL0269
細(xì)胞種屬： 人源細(xì)胞系
細(xì)胞別名： RT4; RT4P
發(fā)貨規(guī)格： 1×10?
供體信息： 63歲白人男性
腫瘤類型： 移行細(xì)胞狀瘤
組織來源： 膀胱
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背景信息
  • 種屬： 人源細(xì)胞系
  • 組織來源： 膀胱
  • 腫瘤類型： 移行細(xì)胞狀瘤
  • 供體信息： 63歲白人男性
  • 背景描述： 1968 年，一名 63 歲男性的復(fù)發(fā)性高分化移行性膀胱移行狀腫瘤（臨床分期 T2，組織學(xué)分級 G1,2 年前用金顆粒治療，10 個月后復(fù)發(fā)后透熱治療）
培養(yǎng)建議
  • 生長形態(tài)： 上皮細(xì)胞樣
  • 生長特性： 貼壁
  • 培養(yǎng)條件： McCOY's5A+10%FBS+1%P/S
  • 凍存條件： 90%FBS+10%DMSO
  • 血清使用等級： 標(biāo)準(zhǔn)
  • 傳代方式： 胰酶消化
  • 倍增時間： 66~80h
  • 傳代比例： 胰酶消化
  • 換液頻率： 每周2~3次
  • 生物安全等級： 1
  • 培養(yǎng)注意事項(xiàng)：
其他信息
  • 蛋白表達(dá)： p53 positive
  • 同工酶： AK-1, 1；ES-D, 1-2；G6PD, B；GLO-I, 1-2；Me-2, 1；PGM1, 1-2；PGM3, 1-2
  • 致瘤性： Yes, in cheek pouch of steroid-treated hamsters
  • 抗原表達(dá)： HLA A25(10), A3, B12, Cw3; Blood Type O
  • 核型： modal number = 49; range = 42 to 55. The cell line is aneuploid human male, with chromosome counts in the near-diploid range. However, the near-tetraploid population may become predominant within very few passages. The 48 and 49 chromosome karyotypes had a single X and a single Y chromosome. Three of the karyotypes at higher ploidy had two X chromosomes and a single Y chromosome. Four marker chromosomes were identified: del(6)(q21), del(10)(p11), 14p+, 12p+. Marker chromosome M4 was not seen in the karyotypes prepared from higher ploidy metaphases.
人膀胱移行細(xì)胞乳頭瘤細(xì)胞
Catalog No CL0269
Product information
  • 蛋白表達(dá)： p53 positive
  • 同工酶： AK-1, 1；ES-D, 1-2；G6PD, B；GLO-I, 1-2；Me-2, 1；PGM1, 1-2；PGM3, 1-2
  • 致瘤性： Yes, in cheek pouch of steroid-treated hamsters
  • 抗原表達(dá)： HLA A25(10), A3, B12, Cw3; Blood Type O
  • 核型： modal number = 49; range = 42 to 55. The cell line is aneuploid human male, with chromosome counts in the near-diploid range. However, the near-tetraploid population may become predominant within very few passages. The 48 and 49 chromosome karyotypes had a single X and a single Y chromosome. Three of the karyotypes at higher ploidy had two X chromosomes and a single Y chromosome. Four marker chromosomes were identified: del(6)(q21), del(10)(p11), 14p+, 12p+. Marker chromosome M4 was not seen in the karyotypes prepared from higher ploidy metaphases.
Hunan UPT Biotechnology Co.,Ltd
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