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首頁 > 細胞 > 人源細胞系 > 人子宮頸鱗癌細胞(SiHa)
人子宮頸鱗癌細胞(SiHa)
￥800.00元
細胞貨號： CL0280
細胞種屬： 人源細胞系
細胞別名： Siha; SIHA
發貨規格： 1×10?
供體信息： 55歲亞洲女性
腫瘤類型： 人瘤病毒相關性宮頸鱗狀細胞癌
組織來源： 子宮頸
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背景信息
  • 種屬： 人源細胞系
  • 組織來源： 子宮頸
  • 腫瘤類型： 人瘤病毒相關性宮頸鱗狀細胞癌
  • 供體信息： 55歲亞洲女性
  • 背景描述： SiHa細胞是一種人宮頸鱗狀細胞癌細胞系，幾十年來已廣泛用于研究。它們是從一名 55 歲的日本女性鱗狀細胞癌患者的原發性子宮活檢片段中分離出來的。由于其獨特的遺傳特征，該細胞系對研究宮頸癌和其他相關疾病的研究人員非常感興趣。
培養建議
  • 生長形態： 上皮細胞樣
  • 生長特性： 貼壁
  • 培養條件： DMEM+10%FBS+1%P/S
  • 凍存條件： 90%FBS+10%DMSO
  • 血清使用等級： 標準
  • 傳代方式： 胰酶消化
  • 倍增時間： 20h
  • 傳代比例： 胰酶消化
  • 換液頻率： 每周2~3次
  • 生物安全等級： 1
  • 培養注意事項：
其他信息
  • 同工酶： AK-1,1；ES-D, 2；G6PD, B；GLO-I, 2；Me-2, 1；PGM1, 1；PGM3, 1
  • 基因表達： p53+; pRB+
  • 致瘤性： Yes, in nude mice; forms poorly differentiated epidermoid carcinoma (grade III)
  • 核型： modal number = 69; range = 51 to 72.This is a hypertriploid human cell line with the modal chromosome number of 71, occurring in 24% of cells. Most cells had the chromosome numbers distributed between 69 and 72. Polyploid cells occurred at 7.6%. Fifteen or more marker chromosomes were common to most cells. Among them are dup(2) (q22q31) and del(2) (q31) which probably resulted from the balanced translocation between two N2s. Most cells had two copies of del(2). M2 is an A3-sized acrocentric. M13 is a minute submetacentric with 1-3 copies per cell. Origins of both M2 and M13 are not identified. There were two copies of normal X chromosomes. N2 was absent and probably was replaced by dup(2) and del(2).
人子宮頸鱗癌細胞
Catalog No CL0280
Product information
  • 同工酶： AK-1,1；ES-D, 2；G6PD, B；GLO-I, 2；Me-2, 1；PGM1, 1；PGM3, 1
  • 基因表達： p53+; pRB+
  • 致瘤性： Yes, in nude mice; forms poorly differentiated epidermoid carcinoma (grade III)
  • 核型： modal number = 69; range = 51 to 72.This is a hypertriploid human cell line with the modal chromosome number of 71, occurring in 24% of cells. Most cells had the chromosome numbers distributed between 69 and 72. Polyploid cells occurred at 7.6%. Fifteen or more marker chromosomes were common to most cells. Among them are dup(2) (q22q31) and del(2) (q31) which probably resulted from the balanced translocation between two N2s. Most cells had two copies of del(2). M2 is an A3-sized acrocentric. M13 is a minute submetacentric with 1-3 copies per cell. Origins of both M2 and M13 are not identified. There were two copies of normal X chromosomes. N2 was absent and probably was replaced by dup(2) and del(2).
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