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首頁 > 細(xì)胞 > 人源細(xì)胞系 > 人皮膚惡性黑色素瘤細(xì)胞(SK-MEL-28)
人皮膚惡性黑色素瘤細(xì)胞(SK-MEL-28)
￥800.00元
細(xì)胞貨號(hào)： CL0284
細(xì)胞種屬： 人源細(xì)胞系
細(xì)胞別名： SK-Mel-28; SK.MEL.28; SK-MEL 28; SK MEL-28; SK MEL 28; SK Mel 28; SKMel-28; SKMEL-28; SK-MEL28; SK-Mel28; SK Mel28; SKMEL28; SKMel28; SKmel28; SKML-28; SK28; AU-Mel; P-36; P36
發(fā)貨規(guī)格： 1×10?
供體信息： 51歲男性
腫瘤類型： 皮膚黑色素瘤
組織來源： 皮膚
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背景信息
  • 種屬： 人源細(xì)胞系
  • 組織來源： 皮膚
  • 腫瘤類型： 皮膚黑色素瘤
  • 供體信息： 51歲男性
  • 背景描述： 該細(xì)胞系是由 T.Takahashi 及其同事從一名種族不明的 51 歲男性的腋窩淋巴結(jié)中建立的，他們?cè)谝幌盗泻谏亓鱿抵蟹蛛x出該細(xì)胞系。
培養(yǎng)建議
  • 生長形態(tài)： 多角形
  • 生長特性： 貼壁
  • 培養(yǎng)條件： RPMI-1640+10%FBS+1%P/S
  • 凍存條件： 90%FBS+10%DMSO
  • 血清使用等級(jí)： 標(biāo)準(zhǔn)
  • 傳代方式： 胰酶消化
  • 倍增時(shí)間： 27h
  • 傳代比例： 胰酶消化
  • 換液頻率： 每周2~3次
  • 生物安全等級(jí)： 1
  • 培養(yǎng)注意事項(xiàng)：
其他信息
  • 蛋白表達(dá)： p53 positive
  • 同工酶： PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1-2, GLO-1, 2, G6PD, B
  • 致瘤性： Yes, in nude mice. Forms malignant melanoma (large round cell type)
  • 抗原表達(dá)： Blood Type A; Rh+; HLA A11, A26, B40, DRw4
  • 突變信息： BRAF V600E mut: V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot), N-Ras wt
  • 核型： modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell.
人皮膚惡性黑色素瘤細(xì)胞
Catalog No CL0284
Product information
  • 蛋白表達(dá)： p53 positive
  • 同工酶： PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1-2, GLO-1, 2, G6PD, B
  • 致瘤性： Yes, in nude mice. Forms malignant melanoma (large round cell type)
  • 抗原表達(dá)： Blood Type A; Rh+; HLA A11, A26, B40, DRw4
  • 突變信息： BRAF V600E mut: V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot), N-Ras wt
  • 核型： modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell.
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