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首頁 > 細胞 > 人源細胞系 > 人結腸癌細胞(SW620)
人結腸癌細胞(SW620)
￥800.00元
細胞貨號： CL0305
細胞種屬： 人源細胞系
細胞別名： SW-620; SW 620; SW.620
發貨規格： 1×10?
供體信息： 51歲白人男性
腫瘤類型： 結直腸腺癌
組織來源： 大腸
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背景信息
  • 種屬： 人源細胞系
  • 組織來源： 大腸
  • 腫瘤類型： 結直腸腺癌
  • 供體信息： 51歲白人男性
  • 背景描述： SW-620細胞系來自一名患有Dukes-C型結直腸癌的51歲男性的大腸，是結直腸癌的關鍵研究模型，特別是用于癌癥生物標志物、化療和轉移性癌細胞的研究。SW-620細胞是研究細胞凋亡和抗失巢凋亡機制的關鍵，失巢凋亡是一種對防止轉移至關重要的程序性細胞死亡形式。利用SW-620結腸癌細胞的研究已經深入到蛋白質組分析中，以了解不同條件下（如缺氧）的蛋白質組變化。低氧SW620細胞表現出特定的蛋白質組適應，這有助于化療耐藥性。
培養建議
  • 生長形態： 上皮細胞樣
  • 生長特性： 貼壁
  • 培養條件： RPMI-1640+10%FBS+1%P/S
  • 凍存條件： 90%FBS+10%DMSO
  • 血清使用等級： 標準
  • 傳代方式： 胰酶消化
  • 倍增時間： 30~37h
  • 傳代比例： 胰酶消化
  • 換液頻率： 每周2~3次
  • 生物安全等級： 1
  • 培養注意事項：
其他信息
  • 蛋白表達： keratin
  • 同工酶： ES-D, 1；G6PD, B；PEP-D, 1；PGD, A；PGM1, 2；PGM3, 1
  • 基因表達： carcinoembryonic antigen (CEA) 0.15 ng/106 cells/10 days; transforming growth factor alpha; matrilysin; CSAp negative; and colon antigen 3 negative; keratin positive by immunoperoxidase staining; c-myc; K-ras; H-ras; N-ras; Myb; sis; fos
  • 致癌基因： myc+; myb+; ras+; fos+; sis+; p53+; abl-; ros-; src-
  • 致瘤性： Yes, in nude mice。(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
  • 抗原表達： Blood type A; Rh+
  • 突變信息： There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.
  • 核型： modal number = 50; range = 45 to 53. The stemline chromosome number is hyperdiploid with the 2S component occurring at 12% and 11 marker chromosomes were common to S metaphases. One of the 11 markers [7 (7p; 11p)] was disomic., M1 was probably an HSR but the origin of the chromosome could not be identified.
人結腸癌細胞
Catalog No CL0305
Product information
  • 蛋白表達： keratin
  • 同工酶： ES-D, 1；G6PD, B；PEP-D, 1；PGD, A；PGM1, 2；PGM3, 1
  • 基因表達： carcinoembryonic antigen (CEA) 0.15 ng/106 cells/10 days; transforming growth factor alpha; matrilysin; CSAp negative; and colon antigen 3 negative; keratin positive by immunoperoxidase staining; c-myc; K-ras; H-ras; N-ras; Myb; sis; fos
  • 致癌基因： myc+; myb+; ras+; fos+; sis+; p53+; abl-; ros-; src-
  • 致瘤性： Yes, in nude mice。(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
  • 抗原表達： Blood type A; Rh+
  • 突變信息： There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.
  • 核型： modal number = 50; range = 45 to 53. The stemline chromosome number is hyperdiploid with the 2S component occurring at 12% and 11 marker chromosomes were common to S metaphases. One of the 11 markers [7 (7p; 11p)] was disomic., M1 was probably an HSR but the origin of the chromosome could not be identified.
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