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首頁(yè) > 細(xì)胞 > 人源細(xì)胞系 > 人骨肉瘤細(xì)胞(U-2OS)
人骨肉瘤細(xì)胞(U-2OS)
￥800.00元
細(xì)胞貨號(hào)： CL0326
細(xì)胞種屬： 人源細(xì)胞系
細(xì)胞別名： U-2 OS; U-2OS; U-2-OS; U2-OS; U20-S; U20S; 2T
發(fā)貨規(guī)格： 1×10?
供體信息： 15歲白人女性
腫瘤類型： 骨 肉瘤
組織來(lái)源： 脛骨
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背景信息
  • 種屬： 人源細(xì)胞系
  • 組織來(lái)源： 脛骨
  • 腫瘤類型： 骨 肉瘤
  • 供體信息： 15歲白人女性
  • 背景描述： U2OS 細(xì)胞是一種源自人類骨肉瘤患者的骨肉瘤細(xì)胞系，在癌癥研究中發(fā)揮著重要作用，尤其是在骨癌研究中。U2OS 細(xì)胞廣泛用于癌癥研究、藥物開發(fā)、細(xì)胞凋亡研究、遺傳研究和放射腫瘤學(xué)研究。U2OS細(xì)胞的價(jià)值在于它們用于研究細(xì)胞凋亡和耐藥性，這對(duì)于制造小分子抑制劑和類似治療劑至關(guān)重要。
培養(yǎng)建議
  • 生長(zhǎng)形態(tài)： 上皮細(xì)胞樣
  • 生長(zhǎng)特性： 貼壁
  • 培養(yǎng)條件： McCoy's5A+10%FBS+1%P/S
  • 凍存條件： 90%FBS+10%DMSO
  • 血清使用等級(jí)： 標(biāo)準(zhǔn)
  • 傳代方式： 胰酶消化
  • 倍增時(shí)間： 25~30h
  • 傳代比例： 胰酶消化
  • 換液頻率： 每周2~3次
  • 生物安全等級(jí)： 1
  • 培養(yǎng)注意事項(xiàng)：
其他信息
  • 蛋白表達(dá)： Osteosarcoma derived growth factor (ODGF)
  • 受體表達(dá)： Insulin-like growth factor I (IGF-I), insulin-like growth factor II (IGF-I), Osteosarcoma derived growth factor (ODGF)
  • 同工酶： PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0082
  • 抗原表達(dá)： Blood Type A, Rh+, HLA A2, Aw30, B12, Bw35, B40(+/-)
  • 核型： Cell line U-2 OS is chromosomally highly altered, with chromosome counts in the hypertriploid range. We did not find the hypodiploid cell population described by J. Ponten, et al., Instead, most of the population has slightly higher counts than first described. Very few normal chromosomes are present, but a high number of stable marker chromosomes are identified., Different chromosomal rearrangements involving the same chromosomes (N1, N7, N9, and N11 particularly), are seen. Twenty-two markers are found including: t(9qter--->9q21::1p36--->1p::?), 7p+, iso(17q), t(15q;?), 4q+, del(3)(q21), 5q(aberrant) and others.
人骨肉瘤細(xì)胞
Catalog No CL0326
Product information
  • 蛋白表達(dá)： Osteosarcoma derived growth factor (ODGF)
  • 受體表達(dá)： Insulin-like growth factor I (IGF-I), insulin-like growth factor II (IGF-I), Osteosarcoma derived growth factor (ODGF)
  • 同工酶： PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0082
  • 抗原表達(dá)： Blood Type A, Rh+, HLA A2, Aw30, B12, Bw35, B40(+/-)
  • 核型： Cell line U-2 OS is chromosomally highly altered, with chromosome counts in the hypertriploid range. We did not find the hypodiploid cell population described by J. Ponten, et al., Instead, most of the population has slightly higher counts than first described. Very few normal chromosomes are present, but a high number of stable marker chromosomes are identified., Different chromosomal rearrangements involving the same chromosomes (N1, N7, N9, and N11 particularly), are seen. Twenty-two markers are found including: t(9qter--->9q21::1p36--->1p::?), 7p+, iso(17q), t(15q;?), 4q+, del(3)(q21), 5q(aberrant) and others.
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