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首頁 > 細胞 > 人源細胞系 > 人腎透明細胞癌皮膚轉移細胞(caki-1)
人腎透明細胞癌皮膚轉移細胞(caki-1)
￥800.00元
細胞貨號： CL0344
細胞種屬： 人源細胞系
細胞別名： CAKI-1; CaKi-1; caki-1; CAKI.1; CAKI 1; CAKI1; Caki1
發貨規格： 1×10?
供體信息： 49歲白人男性
腫瘤類型： 透明細胞癌
組織來源： 腎
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背景信息
  • 種屬： 人源細胞系
  • 組織來源： 腎
  • 腫瘤類型： 透明細胞癌
  • 供體信息： 49歲白人男性
  • 背景描述： Caki-1 細胞系來源于人腎透明細胞癌的轉移部位。Caki-1 細胞由位于男性患者腎靜脈壁的腫瘤建立而成，通常用于腎癌生物學研究，特別是在了解透明細胞腎細胞癌 （ccRCC） 的潛在機制方面。該細胞系在形態上呈上皮樣，并表現出強大的體外生長特性，使其適用于各種實驗技術，包括藥物篩選和分子生物學研究。
培養建議
  • 生長形態： 上皮細胞樣
  • 生長特性： 貼壁
  • 培養條件： McCoy's5A+10%FBS+1%P/S
  • 凍存條件： 90%FBS+10%DMSO
  • 血清使用等級： 標準
  • 傳代方式： 胰酶消化
  • 倍增時間： 40~60h
  • 傳代比例： 胰酶消化
  • 換液頻率： 每周2~3次
  • 生物安全等級： 1
  • 培養注意事項：
其他信息
  • 致瘤性： Yes, in nude mice
  • 抗原表達： Blood Type O; Rh-; HLA A9, B12, Bw35
  • 核型： modal number = 68; range = 63 to 71.The cell line is aneuploid human, with chromosome counts in the triploid range. The Y chromosome is absent; however, loss of the Y chromosome is not unusual from male tumor cell lines. All normal autosomes except chromosome N9 and N19 are represented, usually by two or three copies. Chromosome N9 is recognized as a marker chromosome (M1) that is usually trisomic. Normal chromosome N5 is and chromosomes N10 and N16 tend to be over-represented with respect to the copy number of other normal chromosomes. Thirteen marker chromosomes are identified: 9q+, t(1p;?), t(1qter>1q21::20), t(1q17q), der(11)t(3;11)(q21;q14), 19q+, 4q+, 4p+ and others. The chromosome counts and general cytogenetic features are in keeping with those described by J. Fogh, et al., J. Natl. Cancer Inst. (Bethesda) 58: 209, 1977.
人腎透明細胞癌皮膚轉移細胞
Catalog No CL0344
Product information
  • 致瘤性： Yes, in nude mice
  • 抗原表達： Blood Type O; Rh-; HLA A9, B12, Bw35
  • 核型： modal number = 68; range = 63 to 71.The cell line is aneuploid human, with chromosome counts in the triploid range. The Y chromosome is absent; however, loss of the Y chromosome is not unusual from male tumor cell lines. All normal autosomes except chromosome N9 and N19 are represented, usually by two or three copies. Chromosome N9 is recognized as a marker chromosome (M1) that is usually trisomic. Normal chromosome N5 is and chromosomes N10 and N16 tend to be over-represented with respect to the copy number of other normal chromosomes. Thirteen marker chromosomes are identified: 9q+, t(1p;?), t(1qter>1q21::20), t(1q17q), der(11)t(3;11)(q21;q14), 19q+, 4q+, 4p+ and others. The chromosome counts and general cytogenetic features are in keeping with those described by J. Fogh, et al., J. Natl. Cancer Inst. (Bethesda) 58: 209, 1977.
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