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首頁 > 細胞 > 人源細胞系 > 人膀胱移行細胞癌(J82)
人膀胱移行細胞癌(J82)
￥800.00元
細胞貨號： CL0347
細胞種屬： 人源細胞系
細胞別名： J-82; J 82; J82COT; J82 COT; J82 CO'T; J82/WT
發貨規格： 1×10?
供體信息： 58歲男性歐洲人
腫瘤類型： 膀胱癌
組織來源： 膀胱
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背景信息
  • 種屬： 人源細胞系
  • 組織來源： 膀胱
  • 腫瘤類型： 膀胱癌
  • 供體信息： 58歲男性歐洲人
  • 背景描述： 該細胞源自一名58歲患有膀胱移行細胞癌的白人男性。胞內無橋粒，有大量粗面內質網及突出絨毛，表達ras基因。
培養建議
  • 生長形態： 上皮細胞樣
  • 生長特性： 貼壁
  • 培養條件： MEM+10%FBS+1%P/S
  • 凍存條件： 90%FBS+10%DMSO
  • 血清使用等級： 標準
  • 傳代方式： 胰酶消化
  • 倍增時間： 23h
  • 傳代比例： 胰酶消化
  • 換液頻率： 每周2~3次
  • 生物安全等級： 1
  • 培養注意事項：
其他信息
  • 同工酶： AK-1, 1；ES-D, 1；G6PD, B；GLO-I, 2；Me-2, 1-2；PGM1, 1；PGM3, 2
  • 致癌基因： Contains the ras (H-ras) oncogene
  • 致瘤性： Yes，in nude mice
  • 抗原表達： HLA A2, Aw32, B5, B12, Cw5, Blood Type A
  • 核型： The cell line is aneuploid human male (XY), with most chromosome counts in the triploid range. However, the chromosome count range is quite broad, extending from hyperdiploid to hexaploid. Normal chromosomes N11 and N20 are under-represented with respect to the other normal chromosomes: altered forms of these two chromosomes are prominent as marker chromosomes. Chromosome N13 tends towards over-representation. Five marker chromosomes are noted: 20q+, 11q+, 8p+, del(1)(q31), 5p+(HSR). One of these, 20q, is identical to marker chromosome M1 of C. O'Toole, et al., Br. J. Cancer 38: 64, 1978. The remainder are not as clearly related to the original marker chromosomes noted by those authors.
人膀胱移行細胞癌
Catalog No CL0347
Product information
  • 同工酶： AK-1, 1；ES-D, 1；G6PD, B；GLO-I, 2；Me-2, 1-2；PGM1, 1；PGM3, 2
  • 致癌基因： Contains the ras (H-ras) oncogene
  • 致瘤性： Yes，in nude mice
  • 抗原表達： HLA A2, Aw32, B5, B12, Cw5, Blood Type A
  • 核型： The cell line is aneuploid human male (XY), with most chromosome counts in the triploid range. However, the chromosome count range is quite broad, extending from hyperdiploid to hexaploid. Normal chromosomes N11 and N20 are under-represented with respect to the other normal chromosomes: altered forms of these two chromosomes are prominent as marker chromosomes. Chromosome N13 tends towards over-representation. Five marker chromosomes are noted: 20q+, 11q+, 8p+, del(1)(q31), 5p+(HSR). One of these, 20q, is identical to marker chromosome M1 of C. O'Toole, et al., Br. J. Cancer 38: 64, 1978. The remainder are not as clearly related to the original marker chromosomes noted by those authors.
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