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首頁(yè) > 細(xì)胞 > 人源細(xì)胞系 > 人子宮頸表皮癌細(xì)胞(MS751)
人子宮頸表皮癌細(xì)胞(MS751)
￥800.00元
細(xì)胞貨號(hào)： CL0410
細(xì)胞種屬： 人源細(xì)胞系
細(xì)胞別名： MS-751; MS 751
發(fā)貨規(guī)格： 1×10?
供體信息： 47歲白人女性
腫瘤類型： 人瘤病毒相關(guān)性宮頸鱗狀細(xì)胞癌
組織來(lái)源： 子宮頸
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背景信息
  • 種屬： 人源細(xì)胞系
  • 組織來(lái)源： 子宮頸
  • 腫瘤類型： 人瘤病毒相關(guān)性宮頸鱗狀細(xì)胞癌
  • 供體信息： 47歲白人女性
  • 背景描述： MS751 是一種具有上皮形態(tài)的細(xì)胞系，是從一名 47 歲的白人女性表皮樣癌患者的子宮中分離出來(lái)的。本產(chǎn)品在癌癥和傳染病研究中有應(yīng)用。
培養(yǎng)建議
  • 生長(zhǎng)形態(tài)： 上皮細(xì)胞樣
  • 生長(zhǎng)特性： 貼壁
  • 培養(yǎng)條件： MEM+10%FBS+1%P/S
  • 凍存條件： 90%FBS+10%DMSO
  • 血清使用等級(jí)： 標(biāo)準(zhǔn)
  • 傳代方式： 胰酶消化
  • 倍增時(shí)間： 57.6h
  • 傳代比例： 胰酶消化
  • 換液頻率： 每周2~3次
  • 生物安全等級(jí)： 1
  • 培養(yǎng)注意事項(xiàng)：
其他信息
  • 同工酶： AK-1, 1；ES-D, 1；G6PD, B；GLO-I, 1-2；Me-2, 1；PGM1, 1-2；PGM3, 1-2
  • 基因表達(dá)： MS751 cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. More recently, it has been shown that MS751 cells contain a partial HPV-45 genome, and that HPV-45 sequences from the E6/E7 region are expressed as poly(A)+ RNA.
  • 致瘤性： Yes, in nude mice; forms poorly differentiated epidermoid carciNoma (grade III).
  • 抗原表達(dá)： Blood Type AB; Rh+
  • 核型： model number = 39; range = 38 to 41. This is a hypodiploid human cell line with the modal chromosome number of 39, occurring in 62% of cells. The rate of cells with higher ploidies was 16%. Sixteen markers were found in single copies in all cells. They include del(2) (q34), del(8) (p21), 14p+, der(X)t(X;14) (q28;q13), t(3q8q) and eleven others. A few different markers, all of which had 11qter--11p13 [der(11)] in common, were found. These markers appeared to be mutually exclusive, each cell having one, and only one, marker at a time. Normal X, N8, N11, N21 and N22 were absent. N1, N10, N15, and N16 were paired, and other normal chromosomes were single.
人子宮頸表皮癌細(xì)胞
Catalog No CL0410
Product information
  • 同工酶： AK-1, 1；ES-D, 1；G6PD, B；GLO-I, 1-2；Me-2, 1；PGM1, 1-2；PGM3, 1-2
  • 基因表達(dá)： MS751 cells have been reported to contain human papilloma virus 18 (HPV-18) sequences. More recently, it has been shown that MS751 cells contain a partial HPV-45 genome, and that HPV-45 sequences from the E6/E7 region are expressed as poly(A)+ RNA.
  • 致瘤性： Yes, in nude mice; forms poorly differentiated epidermoid carciNoma (grade III).
  • 抗原表達(dá)： Blood Type AB; Rh+
  • 核型： model number = 39; range = 38 to 41. This is a hypodiploid human cell line with the modal chromosome number of 39, occurring in 62% of cells. The rate of cells with higher ploidies was 16%. Sixteen markers were found in single copies in all cells. They include del(2) (q34), del(8) (p21), 14p+, der(X)t(X;14) (q28;q13), t(3q8q) and eleven others. A few different markers, all of which had 11qter--11p13 [der(11)] in common, were found. These markers appeared to be mutually exclusive, each cell having one, and only one, marker at a time. Normal X, N8, N11, N21 and N22 were absent. N1, N10, N15, and N16 were paired, and other normal chromosomes were single.
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